CD117-Antikörperreagenz für die Immunhistochemie

【Product name】

IHC Antibody --CD117

【Packing specification】

【Intended use】

For Research use only. CD117 Antibody reagent is intended for use to qualitatively identify CD117 by microscopy in sections of FFPE tissue using immunohistochemical detection system.

【Principle】

Stem cell factor receptor (CD117, the gene product of the c-kit protooncogene) and its ligand, stem cell factor (also named c-kit ligand, mast cell growth factor), play essential roles in gametogenesis, melanogenesis and hematopoiesis. It is a transmembrane tyrosine kinase that is expressed on endothelial cells, mast cells, megakaryocytes, stem cells and multiple embryonic cells, such as melanoblasts and primordial germ cells. Add the primary antibody to bind the antigen on tissue sections, and then use HRP labeled secondary antibody binding primary antibody to form the secondary antibody-primary antibody-antigen complex. When DAB chromogenic solution is added, HRP reacts with enzyme substrate to produce brown insoluble reaction product, which indirectly indicating the existence of antigen. 

【Main components】

Immunoglobulin, antibody diluent

【Storage】

Store at 2～8℃ for 18 months。

【Sample requirements】

FFPE tissues are usually cut into sections as thin as 3～5μm with a microtome. These sections are then mounted onto glass slides that are coated with a tissue adhesive.

【Protocol】

1. Sample preparation：Deparaffinize the slides in xylene Ⅰ, Ⅱ, Ⅲ for 5 minutes；Transfer the slides once through 100%, 100%, 95%, 75% alcohols for 2 minutes respectively. Rinse slides with deionized water for 30 seconds.

2. Blocking：Block endogenous peroxidase activity by incubating sections in 3% H₂O₂ solution at room temperature for 5 minutes to block endogenous peroxidase activity. Rinse the slides with deionized water for 30 seconds.

3. Antigen retrieval：Heat the EDTA Antigen retrieval buffer to 100℃. Then place the slides in the boiled buffer and continue to heat for 15～20 min. Naturally cool down for 30 minutes. Rinse the sample with wash buffer.

4. Primary antibody incubation: Drain the slides. Add primary antibody to tissue, incubate at room temperature for 30 minutes. (use antibody diluent or PBS as control). Wash the slides in PBST for 2 times, 5 minutes for each time. If the Primary antibody is concentrated, please dilute it to RTU(ready to use) according to the information on packing. 

5. Secondary antibody: Drain the slides. Add secondary antibody to tissue and incubate at room temperature for 20 minutes. Wash the slides in PBST for 2 times, 5 minutes for each time.

6. DAB：Drain the slides. Add DAB to the tissue and incubate at room temperature for 5 min. Rinse slides with deionized water.

7. Hematoxylin staining：Drain the slides. Add Hematoxylin to the tissue and incubate at room temperature for 5 minutes. Rinse slides with water. Use the acid solution for differentiation. Rinse slides with water.

8. Dehydrieren : Dehydrieren Sie die Objektträger 2 Minuten lang in 75-prozentigem , 95-prozentigem und 100-prozentigem  Alkohol . Trocknen Sie die Objektträger. Beflecktes Gewebe mit einem Deckglas mit Eindeckmedium abdecken. 

【Positive Lokalisierung 】

1.  Positive Lokalisierung :  Zytoplasma/Membran .

2.  Positive Kontrolle :  GIST .

【Vorsichtsmaßnahmen 】

1.  Bitte lesen Sie die Anleitung sorgfältig durch und machen Sie sich vor der Verwendung mit allen Komponenten des Kits vertraut. Befolgen Sie die Anweisungen während des Betriebs genau.

2.  Verwenden Sie das Kit oder die Kit-Komponenten NICHT nach Gebrauch.

3.  Dieses Kit darf nur von geschultem Fachpersonal verwendet werden. Bitte tragen Sie beim Umgang mit den Reagenzien einen geeigneten Laborkittel und Einweghandschuhe.

4.  Vermeiden Sie den Kontakt von Haut, Augen und Schleimhäuten mit den Chemikalien.

5.  Pipettieren Sie NICHT mit dem Mund.

6.  Nicht verwendete Reagenzien, gebrauchte Kits und Abfälle müssen gemäß den örtlichen Vorschriften entsorgt werden.

【Hersteller 】_

Firmenname: Xiamen Talent Biomedical Technology Co., Ltd

Adresse: Nr. 3. und 4. Etage, Gebäude B10, Nr. 2068 Wengjiao Road West, BioMedical Park, Bezirk Haicang, Stadt Xiamen, 36100 China

Tel.: +86 592 6315755            

E-Mail : tlsw@talentbiomedical.com                

Website : www.talentbiomedical.com

【 Symbole 】